Gene Sequence Variation and Genetic Differentiation of Mitochondrial COI in Genus Saperda (Coleoptera:Cerambycidae:Lamiinae)

Volume 6, Issue 1, February 2021     |     PP. 1-12      |     PDF (327 K)    |     Pub. Date: March 7, 2021
DOI: 10.54647/biology18132    172 Downloads     5938 Views  

Author(s)

Xu Wei, Post-Entry Quarantine Station for Tropical Plants of Haikou Customs, Haikou 570311, Hainan Province, China; College of Plant Protection,Southwest University,Chongqing 400716,Chongqing municipality, China;4.Poly Pharm Co. Ltd.haikou 571127,Hainan Province,China
Gan Qinhua, Hainan University,Haikou 570228,Hainan Province,China
Pan Yingwen, Post-Entry Quarantine Station for Tropical Plants of Haikou Customs, Haikou 570311, Hainan Province, China
Cai Bo, Post-Entry Quarantine Station for Tropical Plants of Haikou Customs, Haikou 570311, Hainan Province, China
Ao Su, Post-Entry Quarantine Station for Tropical Plants of Haikou Customs, Haikou 570311, Hainan Province, China
Meng rui, Post-Entry Quarantine Station for Tropical Plants of Haikou Customs, Haikou 570311, Hainan Province, China
Chen Li, College of Plant Protection,Southwest University,Chongqing 400716,Chongqing municipality, China;4.Poly Pharm Co. Ltd.haikou 571127,Hainan Province,China
Pu Jian, Poly Pharm Co. Ltd.haikou 571127,Hainan Province,China

Abstract
In this study，the total DNA of nine species of Saperda (Lopezcolonia) octopunctata (Scopoli, 1772), Saperda (Lopezcolonia) scalaris (Linnaeus, 1758), Saperda interrupta Gebler, Saperda Alberti (Plavilstshikov), Saperda (Saperda) similis Laicharting, 1784, Saperda (Compsidia) populnea (Linnaeus, 1758),Saperda (Saperda) carcharias (Linnaeus, 1758), Saperda (Lopezcolonia) perforata Pallas, 1773 and Saperda ohbayashi were extracted . Two partial sequences of mitochondrial gene and one partial sequence of nuclear gene were amplified. Comparing the COI sequence with the DNA barcode data in GenBank can effectively identify the related species of Saperda. It will be applied to the rapid identification of some species of Saperda in imported wood at ports, and improve the detection rate of plant quarantine.

Keywords
Genus Saperda;mtDNA;COI;detection;sequence analysis

Cite this paper
Xu Wei, Gan Qinhua, Pan Yingwen, Cai Bo, Ao Su, Meng rui, Chen Li, Pu Jian, Gene Sequence Variation and Genetic Differentiation of Mitochondrial COI in Genus Saperda (Coleoptera:Cerambycidae:Lamiinae) , SCIREA Journal of Biology. Volume 6, Issue 1, February 2021 | PP. 1-12. 10.54647/biology18132

References

[ 1 ] Li Jing, An Shiheng, Yin Xinming. Phylogeny of 12 species of Lamiinae beetles based on COI gene sequence. Central China insect research (Vol. 7), 2011:243
[ 2 ] Li Jing, An Shiheng, Zhang Hongfei, et al. Characteristics and phylogeny of mitochondrial Co Ⅱ gene in some species of Lamiinae beetles. Journal of Henan Agricultural University, 2013, 47 (6): 715-721
[ 3 ] Ma Jian, Li Chengde, Deng Liwen, et al. Assisted identification of several poplar stem borer larvae based on COI gene restriction endonuclease. Journal of Northeast Forestry University, 2010, 38 (5): 83-87
[ 4 ] Zheng Sizhu, Zhang Kai, Yang Xiaojun, et al. Comparison of COI genes and preliminary systematic study
[ 5 ] on Monochamus. Acta Entomologica Sinica, 2015, 52 (2): 370-381
[ 6 ] Zhang Shuang, Su Xiaoyu, Huang Dazhuang, et al. Sequence variation and genetic differentiation of mitochondrial COI gene in different geographic populations of Germari cerambycids. Sericultural Science, 2015, 41 (2): 234-238
[ 7 ] Kethidi D R, Roden D B, Ladd T R, et al. Development of SCAR markers for the DNA-based detection of the Asian long-horned beetle, Anoplophora glabripennis (Motschulsky). Arch Insect Bioehem Physiol, 2003, 52(4): 193-204